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Wheather all the plugs are out or not won't make much difference other than being easier on the starter and battery, as coldknock said. But the result will be more accurate if the throttle blades are held wide open, whether FI or carb. therefore letting the combustion chamber load with a full charge from the intake. It will eventually fully load if you turn the motor over enough times, but with a wide open throttle, it should give an accurate reading within 3 revs of the crank.
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